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Purification and Identification of some Anthocyanins from Hibiscus sabdariffa, a Medicinal Plant of the Ivorian Pharmacopeia

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Purification and Identification of some Anthocyanins from Hibiscus sabdariffa, a Medicinal Plant of the Ivorian Pharmacopeia ( purification-and-identification-some-anthocyanins-from-hibis )

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Int. J. Curr. Res. Biosci. Plant Biol. 2015, 2(10): 123-131 International Journal of Current Research in Biosciences and Plant Biology ISSN: 2349-8080 Volume 2 Number 10 (October-2015) pp. 123-131 www.ijcrbp.com Original Research Article Purification and Identification of some Anthocyanins from Hibiscus sabdariffa, a Medicinal Plant of the Ivorian Pharmacopeia Abba P. Obouayeba1*, Eric F. Soumahin2, Moussa Diarrassouba3 and Tanoh H. Kouakou4 1Laboratory of Biochemical Pharmacodynamy, Department of Biosciences, Felix Houphouet- Boigny University of Abidjan, 22 BP 582 Abidjan 22, Cote d’Ivoire 2Laboratory of Plant Physiology, Department of Agroforestry, Jean Lorougnon Guédé University of Daloa, BP 150 Daloa, Cote d’Ivoire 3Section of Life and Earth Sciences, Department of Sciences and Technology, Normal Superior School, 25 BP 663 Abidjan 25, Cote d’Ivoire 4Laboratory of Biology and Crop Improvement, Department of Natural Sciences, Nangui Abrogoua University of Abidjan, 02 BP 801 Abidjan 01, Cote d’Ivoire *Corresponding author. Abstract Keywords Hibiscus sabdariffa is a medicinal and food plant rich in phytochemical compounds which are the source of its biological properties. The present work was conducted in objective to identify the anthocyanins which are medicinally important compounds by the methodes such as CPC and HPLC. The results showed that four Hibiscus anthocyanins were isolated and identified from petals of Hibiscus sabdariffa. It is cyanidin 3-O-sambubioside, delphinidin 3-O- sambubioside cyanidin 3-O-glucoside and delphinidin 3-O-glucoside with cyanidin 3-O-sambubioside and delphinidin 3-O-sambubioside as the major Anthocyanins Cyanidin 3-O-glucoside Cyanidin 3-O-sambubioside Delphinidin 3-O-glucoside Delphinidin 3-O-sambubioside Hibiscus sabdariffa compounds of the petal of this plant. The presence of these phytochemical compounds justifies its uses in folkloric medicines. Introduction In recent times, focus on plant research has increased all over the world and a large body of evidence has collected to show immense potentials of medicinal plants used in various traditional systems. Various medicinal plants have been studied using modern scientific approaches. Ethnobotany and ethnopharmacognosy, the basis of useful knowledge on plants in their relationship with traditional or popular therapeutic uses, constitute a guide for chemical, pharmacological and physiological studies that allow the establishment of a scientific foundation for supposed therapeutic properties. The results from these plants have revealed the potentials of medicinal plants in the area of pharmacology (Kone et al., 2011 and 2012; Obouayeba et al., 2015a and 2014b). Hibiscus sabdariffa L., a member of the Malvaceae family, is an annual dicotyledonous herbaceous shrub A. P. Obouayeba et al. (2015) / Int. J. Curr. Res. Biosci. Plant Biol. 2015, 2(10): 123-131 123

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