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Liquid Chromatography HPLC HPLC: High Performance Liquid Chromatography

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Liquid Chromatography HPLC HPLC: High Performance Liquid Chromatography ( liquid-chromatography-hplc-hplc-high-performance-liquid-chro )

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Liquid Chromatography HPLC HPLC: High Performance Liquid Chromatography • The mechanisms of partitioning and adsorption primarily exploited employed (LC) • HPLC (high pressure utilized) • Analytes – high molecular mass, low volatility Normal phase chromatography - NP Reversed phase chromatography - RP Mobile phase http://www.upchurch.com/TechInfo/hplcIntro.asp# Configurations with more than 2 solvent(s) pumps available Normal phase chromatography: More polar stationary phase and a less polar mobile phase. Bonded-phase silica are used to separate weakly polar compounds Reversed phase chromatography: Non-polar stationary phase and a polar mobile phase Instrument Components Better selectivity based on mobile phase composition http://www.chemistry.adelaide.edu.au/external/soc-rel/content/hplc.htm

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