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CHROMATOGRAPHY SEPARATION METHOD CATEGORISATION

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CHROMATOGRAPHY SEPARATION METHOD CATEGORISATION ( chromatography-separation-method-categorisation )

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CHROMATOGRAPHY fundamentals, methods, instrumentation, trends SEPARATION METHOD CATEGORISATION Zdeněk Šimek Materials for individual study 1. Separations based on distribution of sample components between two phases Masaryk University in Brno Faculty of Science Research Centre for Toxic Compounds in the Environment membrane separations : - electrophoresis - thermal diffusion - mass spectrometry - ultracentrifugation - gravitation RECETOX SEPARATIONS BASED ON DISTRIBUTION OF SAMPLE COMPONENTS BETWEEN TWO PHASES Crucial factor of sample component separation is DISTRIBUTION CONSTANT Types of interfaces separation methods abbreviation K  ( C A ) 1 D(A) (CA)2 measure of component affinity to contacted phases in equilibrium gas - liquid gas - solid distillation liquid – liquid liquid - solid extraction Phase1 Phase 2 organic water adsorbent ion exchanger stationary mobile gas chromatography GLC Equilibrium static and/or dynamic sublimation gas chromatography GSC molecular sieve liquid chromatography LLC, SEC (GPC) fractional crystallization precipitation liquid chromatography molecular sieve, inclusion solution gas solution Ion exchange LSC, IEC 2. Separations based on migration rates differences of sample components Extraction Adsorption Chromatography 34 a) through semi-permeable membrane b) in force field - ultrafiltration (hydrostatic pressure) - reverse osmosis (hydrostatic pressure) - dialysis (concentration differences on membrane sites) - electrodialysis (electric potential differences) 12 force field:

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