logo

CENTRIFUGAL PARTITION CHROMATOGRAPHY: AN OVERVIEW

PDF Publication Title:

CENTRIFUGAL PARTITION CHROMATOGRAPHY: AN OVERVIEW ( centrifugal-partition-chromatography-an-overview )

Next Page View | Return to Search List

Text from PDF Page: 001

Mahesh Kumar G et al. / Vol 4 / Issue 6 / 2014 / 353-360. 353 International Journal of Pharmaceutical Research & Analysis e-ISSN: 2249 – 7781 Print ISSN: 2249 – 779X CENTRIFUGAL PARTITION CHROMATOGRAPHY: AN OVERVIEW G. Mahesh Kumar*, I. Neelam, A. Ajitha, V. Uma Maheshwara Rao *Department of Pharmaceutical Analysis & Quality Assurance, CMR College ofPharmacy, JNTU (H) University, Hyderabad, Andhra Pradesh, India. www.ijpra.com ABSTRACT Centrifugal partition chromatography (CPC) is a new and unique method of liquid-liquid chromatography. CPC enables the separation of components with nearly identical partition ratios, and is performed without the aid of a solid support. The method is used for chromatographic reaction in addition to chromatographic separation. Centrifugal partition chromatography is a type of counter current chromatography, which is an automated liquid-liquid extraction process permitting hundreds of automatic successive extractions. A CPC instrument or a CPC column is a series of channels linked in cascade by ducts and aligned in cartridges or disks in a circle around a rotor; setting the rotor in motion submits this assembly to a constant centrifugal field. The originality of CPC is that it uses any biphasic liquid-liquid system as mobile and stationary phases. The United States branch of Sanki contributed greatly to the worldwide acceptance of the technique. Recent works performed in the Netherlands and in France have, by means of visualization of flow-patterns in CPC channels, contributed to a better knowledge of hydrodynamics and mass transfer phenomena. Centrifugal partition chromatography has wide advantages like No column to replace, no silica to recycle, Low solvent consumption, High flow rate for low run time, High performances. Purity > 99%, recovery>90%,No sample losses, No denaturation, no irreversible adsorption of the sample, Huge application fields from petroleum extract to proteins. Hence it is widely employed in the pharmaceutical industry. Keywords: CPC (Centrifugal partition chromatography), Partition Disc, CPC Column, Mono axial rotor, Centrifugal force, Fraction collector. INTRODUCTION Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. The main aims of this technology are to isolate the maximum amount of a specific molecule at the highest purity, in a minimum of time and without using any silica column or support media [1]. Centrifugal partition chromatography (CPC) is a new and unique method of liquid-liquid chromatography. CPC enables the separation of components with nearly identical partition ratios, and is performed without the aid of a solid support. The method is used for chromatographic reaction in addition to chromatographic separation [2]. CPC is unique because no solid support is used for the stationary phase. Instead, the liquid stationary phase is retained in the column by a combination of centrifugal Corresponding Author:-G. Mahesh Kumar force, the special tortuous column geometry and the density difference between the two liquid phases. The CPC apparatus consists of coil columns "undergoing one particular mode of planetary motion generated by a synchronous coil planet centrifuge". The column contains one or more cartridges in which channels are engraved, attached to a rotor. The less dense stationary phase remains in the column because of the centrifugal force created by the spinning rotor. Consequently, the mobile phase is able to pass through the stationary phase. When a mixture of components is introduced into the mobile phase of the CPC column, it distributes according to the individual components' distribution coefficients while passing through the column. The centrifugal force field applied to the coiled columns promotes the retention of the stationary phase against a continuous flow of mobile phase. The mobile phase flow Email:- gangishetti.mahesh@gmail.com

PDF Image | CENTRIFUGAL PARTITION CHROMATOGRAPHY: AN OVERVIEW

centrifugal-partition-chromatography-an-overview-001

PDF Search Title:

CENTRIFUGAL PARTITION CHROMATOGRAPHY: AN OVERVIEW

Original File Name Searched:

353-360ijpra.pdf

DIY PDF Search: Google It | Yahoo | Bing

Infinity Supercritical 10L CO2 Extractor Supercritical CO2 Hemp Oil Extraction for Full Spectrum Botanical Oil which retains the terpenes and other valuable plant components during extraction. System runs silent with small footprint of 24 inches by 48 inches. More Info

$149,000 Complete CO2 Extraction System: Botanical full spectrum oil extraction. More Info

High Quality Full Spectrum Oil: More Info

Technology for Supercritical CO2 Extraction: More Info

CONTACT TEL: 608-238-6001 Email: greg@infinitysupercritical.com | RSS | AMP