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Anthocyanins purification with Centrifugal Partition Chromatography SCPC-100

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Anthocyanins purification with Centrifugal Partition Chromatography SCPC-100 ( anthocyanins-purification-with-centrifugal-partition-chromat )

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Anthocyanins purification with Centrifugal Partition Chromatography SCPC-100 Introduction Anthocyanins, which are found in high concentration in the black currant, give to the edible berry its characteristic dark purple color. Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. CPC was used to purify few mg of anthocyanins from a crude powder extract. Materials and Methods A Gilson SCPC-100 connects to a Gilson PLC2250 system equipped with 50 mL/min quaternary gradient pump, UV/Vis detector, fraction collector and AGCPC software was used. CPC solvent system is determined with shake flask method to get a Kd= [HPLC peak area of anthocyanin] stat/ [HPLC peak area of anthocyanin] mobile closed to one. Application Note 03 Gilson www.gilson.com/asia asia@gilson.com Page |1

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