ColorPlus Protein Marker

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ColorPlus Protein Marker ( colorplus-protein-marker )

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Contents: 0.1–0.2 mg/ml of each protein in 50 mM NaCl, 63.5 mM Tris-HCl (pH 7.0 @ 25°C), 1 mM Na2EDTA, 2% (w/v) SDS, 40 mM DTT, 0.01% (w/v) phenol red and 10% glycerol. Storage Note: To maximize shelf-life, marker should be boiled upon receipt and aliquotted into single-use tubes. Store at –20°C. Suggested Protocol for Loading a Sample (2): 1. Mix ColorPlus Protein Marker. Bring the desired amount of the ColorPlus Prestained Protein Marker over to a separate tube. For blotting: use 6 μl for mini-gels and 12 μl for full length gels. For visualizing during electrophoresis: use 15 μl for mini-gels and 30 μl for full length gels. 2. Heat the marker to 95–100°C for 3–5 minutes. If the marker has been already boiled upon receipt, don't heat again, directly go to Step 3. 3. After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells. Contents: 0.1–0.2 mg/ml of each protein in 50 mM NaCl, 63.5 mM Tris-HCl (pH 7.0 @ 25°C), 1 mM Na2EDTA, 2% (w/v) SDS, 40 mM DTT, 0.01% (w/v) phenol red and 10% glycerol. Storage Note: To maximize shelf-life, marker should be boiled upon receipt and aliquotted into single-use tubes. Store at –20°C. Suggested Protocol for Loading a Sample (2): 1. Mix ColorPlus Protein Marker. Bring the desired amount of the ColorPlus Prestained Protein Marker over to a separate tube. For blotting: use 6 μl for mini-gels and 12 μl for full length gels. For visualizing during electrophoresis: use 15 μl for mini-gels and 30 μl for full length gels. 2. Heat the marker to 95–100°C for 3–5 minutes. If the marker has been already boiled upon receipt, don't heat again, directly go to Step 3. 3. After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells. kDa 175 80 58 46 30 23 17 7 ColorPlus Prestained Protein Marker, Broad Range ColorPlus Prestained Protein Marker, Broad Range (7–175 kDa) PROTEIN MBP-β-galactosidase1 MBP -truncated-β-galactosidase1 MBP-CBD1 CBD-Mxe Intein-2CBD1 CBD-Mxe Intein1 CBD-E. coli par1 Lysozyme Aprotinin SOURCE E. coli E. coli E. coli E. coli E. coli E. coli chicken egg white bovine lung APPARENT MW (kDa) 175 80 58 46 30 23 17 7 P7709S 022120613061 P7709S 175 mini-gel lanes 1.05 ml Store at –20°C 1-800-632-7799 info@neb.com w w w. n e b. c o m Lot: 0221206 Exp: 6/13 1 MBP = maltose-binding protein. MBP-β-galactosidase = fusion of MBP and β-galactosidase. MBP-truncated-β-galactosidase = fusion of MBP and a truncated β-galactosidase. MBP-CBD = fusion of MBP and chitin binding domain. CBD-Mxe Intein-2CBD = fusion of the chitin binding domain, Mxe Intein followed by two chitin binding domains. CBD-E. coli par = fusion of the chitin binding domain followed by and E. coli parvulin-like protein. Note: Apparent molecular weights of every lot are determined on Invitrogen Novex 10–20% Tris-glycine SDS PAGE gels using NEB's Protein Ladder (NEB #P7703). Description: ColorPlus Prestained Protein Marker, Broad Range includes a mixture of purified proteins covalently coupled to colored dye that resolves to 8 bands of even intensity, including one orange and one green reference band for easy orientation when electrophoresed (1). The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins (1). For precise molecular weight determinations, use NEB's unstained Protein Marker, Broad Range (NEB #P7702) or Protein Ladder (NEB #P7703) in addition to the prestained marker. 10–20% SDS PAGE kDa 175 80 58 46 30 23 17 7 ColorPlus Prestained Protein Marker, Broad Range (See other side) CERTIFICATE OF ANALYSIS ColorPlus Prestained Protein Marker, Broad Range (7–175 kDa) PROTEIN MBP-β-galactosidase1 MBP -truncated-β-galactosidase1 MBP-CBD1 CBD-Mxe Intein-2CBD1 CBD-Mxe Intein1 CBD-E. coli par1 Lysozyme Aprotinin SOURCE E. coli E. coli E. coli E. coli E. coli E. coli chicken egg white bovine lung APPARENT MW (kDa) 175 80 58 46 30 23 17 7 P7709S 022120613061 P7709S 175 mini-gel lanes 1.05 ml Store at –20°C 1-800-632-7799 info@neb.com w w w. n e b. c o m Lot: 0221206 Exp: 6/13 1 MBP = maltose-binding protein. MBP-β-galactosidase = fusion of MBP and β-galactosidase. MBP-truncated-β-galactosidase = fusion of MBP and a truncated β-galactosidase. MBP-CBD = fusion of MBP and chitin binding domain. CBD-Mxe Intein-2CBD = fusion of the chitin binding domain, Mxe Intein followed by two chitin binding domains. CBD-E. coli par = fusion of the chitin binding domain followed by and E. coli parvulin-like protein. Note: Apparent molecular weights of every lot are determined on Invitrogen Novex 10–20% Tris-glycine SDS PAGE gels using NEB's Protein Ladder (NEB #P7703). Description: ColorPlus Prestained Protein Marker, Broad Range includes a mixture of purified proteins covalently coupled to colored dye that resolves to 8 bands of even intensity, including one orange and one green reference band for easy orientation when electrophoresed (1). The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins (1). For precise molecular weight determinations, use NEB's unstained Protein Marker, Broad Range (NEB #P7702) or Protein Ladder (NEB #P7703) in addition to the prestained marker. 10–20% SDS PAGE (See other side) CERTIFICATE OF ANALYSIS

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